Bethyl Laboratories
Rabbit anti-PPP3CA Antibody Affinity Purified
Primary
Catalog #A300-907A
TargetPPP3CA
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
Image 1 of 2
local_shipping
Order within 14 hours and 57 minutes for shipment by Tuesday, November 10
Product Description
PPP3CA encodes the protein phosphatase 2B (PP2B) alpha catalytic subunit. PP2B, also known as calcineurin, is a holoenzyme that is comprised of a catalytic subunit associated with regulatory subunits. PP2B is a calcium regulated enzyme that is activated by calmodulin and participates in the signaling cascades involved in development of the nervous, cardiovascular, and musculoskeletal systems.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetPPP3CA
Antigen Species
Human
Gene ID5530
Gene Symbol
PPP3CA
Gene Name
protein phosphatase 3 catalytic subunit alpha
Uniprot IDQ08209
Protein Name
Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
Alternate Names
calcineurin A alpha • calmodulin-dependent calcineurin A subunit alpha isoform • CALN • CALNA • CALNA1 • CAM-PRP catalytic subunit • CCN1 • CNA1 • IECEE • IECEE1 • PPP2B • protein phosphatase 2B, catalytic subunit, alpha isoform • protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform • protein phosphatase 3 catalytic subunit alpha isozyme • serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to PPP3CA immobilized on solid support.
The epitope recognized by A300-907A maps to a region between residue 1 and 50 of human Protein Phosphatase 3, Catalytic Subunit, Alpha isoform using the numbering given in entry NP_000935.1 (GeneID 5530).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A300-907A · Lot 1 (current lot)Open
Product DatasheetPDF · A300-907A · Lot 1 (current lot)Open
Safety Data SheetPDF · A300-907AOpen
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1