Bethyl Laboratories
Rabbit anti-PRKCBP1 Antibody Affinity Purified
Primary
Catalog #A302-090A
TargetPRKCBP1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
PRKCBP1 was identified in a screen for RACK (receptors for activated C-kinase) family members. RACK proteins function as anchors for activated protein kinase C (PKC) isoenzymes. PRKCBP1 contains one bromodomain, one MYND-type zinc finger, one PHD-type zinc finger, and one PWWP domain.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1136 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetPRKCBP1
Antigen Species
Human
Gene ID23613
Gene Symbol
ZMYND8
Gene Name
zinc finger MYND-type containing 8
Uniprot IDQ9ULU4
Protein Name
Protein kinase C-binding protein 1
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to PRKCBP1 immobilized on solid support.
The epitope recognized by A302-090A maps to a region between residue 1136 and 1186 of human protein kinase C binding protein 1 (zinc finger, MYND-type containing 8) using the numbering given in entry CAI21842.1 (GeneID 23613).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-090A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-090A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-090AOpen
Citations / Publications
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