Bethyl Laboratories
Rabbit anti-PRMT5 Antibody Affinity Purified
Primary
Catalog #A300-850A
TargetPRMT5
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, WB
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Product Description
Protein arginine methylation is a form of post-translational modification that is catalyzed by protein arginine methyltransferases (PRMTs). PRMT5 interacts with the SWI/SNF complexes and methylates histones H3 and H4. This finding suggests that PRMT5 is involved in the structural modification of chromatin and the regulation of gene transcription.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 587 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetPRMT5
Antigen Species
Human
Gene ID10419
Gene Symbol
PRMT5
Gene Name
protein arginine methyltransferase 5
Uniprot IDO14744
Protein Name
Protein arginine N-methyltransferase 5
Product Information
Applications
IHC, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to PRMT5 immobilized on solid support.
The epitope recognized by A300-850A maps to a region between residue 580 and the C-terminus (residue 637) of Protein Arginine Methyltransferase 5 using the numbering given in entry NP_006100.2 (GeneID 10419).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A300-850A · Lot 1 (current lot)Open
Product DatasheetPDF · A300-850A · Lot 1 (current lot)Open
Safety Data SheetPDF · A300-850AOpen
Citations / Publications
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