Bethyl Laboratories
Rabbit anti-PRPF8 Antibody Affinity Purified
Primary
Catalog #A303-921A
TargetPRPF8
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
Pre-mRNA-processing-splicing factor 8 (PRPF8) is a central component of the spliceosome, which may play a role in aligning the pre-mRNA 5'- and 3'-exons for ligation. PRPF8 interacts with U5 snRNA, and with pre-mRNA 5'-splice sites in B spliceosomes and 3'-splice sites in C spliceosomes [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q8TCS8].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1825 and 1875
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetPRPF8
Antigen Species
Human
Gene ID10594
Gene Symbol
PRPF8
Gene Name
pre-mRNA processing factor 8
Uniprot IDQ6P2Q9
Protein Name
Pre-mRNA-processing-splicing factor 8
Alternate Names
220 kDa U5 snRNP-specific protein • apoptosis-regulated protein 1 • apoptosis-regulated protein 2 • HPRP8 • p220 • precursor mRNA processing protein • pre-mRNA-processing-splicing factor 8 • PRP8 • PRP8 homolog • PRP8 pre-mRNA processing factor 8 homolog • PRPC8 • RP13 • SNRNP220 • splicing factor Prp8 • U5 snRNP-specific protein (220 kD), ortholog of S. cerevisiae Prp8p
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to PRPF8 immobilized on solid support.
The epitope recognized by A303-921A maps to a region between residue 1825 and 1875 of human Pre-mRNA-Processing Factor 8 using the numbering given in entry NP_006436.3 (GeneID 10594).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000 (3-8% Tris-acetate gel recommended)
Documents
Product DatasheetPDF · A303-921A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-921A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-921AOpen
Citations / Publications
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