Bethyl Laboratories
Rabbit anti-PSF/SFPQ Antibody Affinity Purified
Primary
Catalog #A301-320A
TargetPSF/SFPQ
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP, WB
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Product Description
PSF/SFPQ is a multifunctional protein know to participate in a variety of nuclear processes and may function to link transcription and pre-mRNA processing. PSF/SFPQ bears an RNA recognition motif (RRM) further indicating its role in post-transcriptional processes. PSF has been shown to associate with p54nrb/NonO to modulate androgen receptor-mediated transcription. Additionally, it has recently been show to associate with XRN2 and p54nrb/NonO to facilitate pre-mRNA 3’ processing and transcription termination.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetPSF/SFPQ
Antigen Species
Human
Gene ID6421
Gene Symbol
SFPQ
Gene Name
splicing factor proline and glutamine rich
Uniprot IDP23246
Protein Name
Splicing factor, proline- and glutamine-rich
Alternate Names
100 kDa DNA-pairing protein • DNA-binding p52/p100 complex, 100 kDa subunit • epididymis secretory sperm binding protein • hPOMp100 • polypyrimidine tract binding protein associated • polypyrimidine tract-binding protein-associated splicing factor • Polypyrimidine tract-binding protein-associated-splicing factor • POMP100 • PPP1R140 • protein phosphatase 1, regulatory subunit 140 • PSF • PTB-associated splicing factor • PTB-associated-splicing factor • splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated) • splicing factor proline/glutamine-rich • splicing factor, proline- and glutamine-rich
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to PSF/SFPQ immobilized on solid support.
The epitope recognized by A301-320A maps to a region between residue 1 and 50 of human Polypyrimidine tract-binding protein-associated-splicing factor (Splicing factor, proline- and glutamine-rich) using the numbering given in entry NP_005057.1 (GeneID 6421).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-320A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-320A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-320AOpen
Citations / Publications
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