Bethyl Laboratories
Rabbit anti-PSF/SFPQ Antibody Affinity Purified
Primary
Catalog #A301-321A
TargetPSF/SFPQ
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
PSF/SFPQ is a multifunctional protein know to participate in a variety of nuclear processes and may function to link transcription and pre-mRNA processing. PSF/SFPQ bears an RNA recognition motif (RRM) further indicating its role in post-transcriptional processes. PSF has been shown to associate with p54nrb/NonO to modulate androgen receptor-mediated transcription. Additionally, it has recently been show to associate with XRN2 and p54nrb/NonO to facilitate pre-mRNA 3’ processing and transcription termination.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 225 and 275
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetPSF/SFPQ
Antigen Species
Human
Gene ID6421
Gene Symbol
SFPQ
Gene Name
splicing factor proline and glutamine rich
Uniprot IDP23246
Protein Name
Splicing factor, proline- and glutamine-rich
Alternate Names
100 kDa DNA-pairing protein • DNA-binding p52/p100 complex, 100 kDa subunit • epididymis secretory sperm binding protein • hPOMp100 • polypyrimidine tract binding protein associated • polypyrimidine tract-binding protein-associated splicing factor • Polypyrimidine tract-binding protein-associated-splicing factor • POMP100 • PPP1R140 • protein phosphatase 1, regulatory subunit 140 • PSF • PTB-associated splicing factor • PTB-associated-splicing factor • splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated) • splicing factor proline/glutamine-rich • splicing factor, proline- and glutamine-rich
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to PSF/SFPQ immobilized on solid support.
The epitope recognized by A301-321A maps to a region between residue 225 and 275 of human Polypyrimidine tract-binding protein-associated-splicing factor (Splicing factor, proline- and glutamine-rich) using the numbering given in entry NP_005057.1 (GeneID 6421).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-321A · Lot 2 (current lot)Open
Product DatasheetPDF · A301-321A · Lot 2 (current lot)Open
Safety Data SheetPDF · A301-321AOpen
Citations / Publications
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