Bethyl Laboratories
Rabbit anti-PSMB2 Antibody Affinity Purified
Primary
Catalog #A303-817A
TargetPSMB2
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP, WB
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Product Description
Proteasome (prosome, macropain) subunit, alpha type, 2 (PSMA2) is a component of the proteasome, a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMA2 is a member of the peptidase T1A family, that is a 20S core alpha subunit [taken from NCBI Entrez Gene (Gene ID: 5683)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 151 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetPSMB2
Antigen Species
Human
Gene ID5690
Gene Symbol
PSMB2
Gene Name
proteasome subunit beta 2
Uniprot IDP49721
Protein Name
Proteasome subunit beta type-2
Alternate Names
HC7-I • macropain subunit C7-I • multicatalytic endopeptidase complex subunit C7-1 • multicatalytic endopeptidase complex subunit C7-I • proteasome (prosome, macropain) subunit, beta type, 2 • proteasome beta 2 subunit • proteasome component C7-I • proteasome subunit beta type-2 • proteasome subunit, beta type, 2 • testicular tissue protein Li 152
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to PSMB2 immobilized on solid support.
The epitope recognized by A303-817A maps to a region between residue 151 and 201 of human Proteasome (prosome, macropain) Subunit, beta type, 2 using the numbering given in entry NP_002785.1 (GeneID 5690).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:1000 - 1:5000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A303-817A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-817A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-817AOpen
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