Rabbit anti-PSME1 Antibody Affinity Purified

Product Details
Specifications
The epitope recognized by A303-858A maps to a region between residue 1 and 50 of human Proteasome (Prosome, Macropain) Activator Subunit 1 (PA28 alpha) using the numbering given in entry NP_006254.1 (GeneID 5720).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PSME1 immobilized on solid support.
The epitope recognized by A303-858A-T maps to a region between residue 1 and 50 of human Proteasome (Prosome, Macropain) Activator Subunit 1 (PA28 alpha) using the numbering given in entry NP_006254.1 (GeneID 5720).
Additional Product Information
PSME1 [proteasome (prosome, macropain) activator subunit 1 (PA28 alpha)] is the alpha subunit of the 11S regulator. The 11S regulator is part of the immunoproteasome, a modified proteasome involved in the processing of class I MHC peptides [taken from NCBI Entrez Gene (Gene ID: 5720)].
Alternate Names
11S regulator complex subunit alpha; 29-kD MCP activator subunit; activator of multicatalytic protease subunit 1; epididymis secretory sperm binding protein Li 129m; HEL-S-129m; IFI5111; IGUP I-5111; interferon gamma up-regulated I-5111 protein; interferon-gamma IEF SSP 5111; interferon-gamma-inducible protein 5111; PA28A; PA28alpha; proteasome (prosome, macropain) activator subunit 1 (PA28 alpha); Proteasome activator 28 subunit alpha; proteasome activator complex subunit 1; REGalpha; REG-alpha
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.