Bethyl Laboratories
Rabbit anti-PTEN IHC Antibody Affinity Purified
Primary
Catalog #IHC-00358
TargetPTEN
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IHC-IF
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Product Description
PTEN (phosphatase and tensin homolog) is a phosphatidylinositol-3,4,5-triphosphate 3-phosphatase that has been identified as a tumor suppressor. PTEN is a dual specificity tyrosine and serine/threonine phosphatase that preferentially dephosphorylates phosphoinositide substrates and negatively regulates the AKT/PKB signaling pathway. Through its action, PTEN has been shown to modulate cell cycle progression, cell survival, cell migration, cell adhesion, and insulin signaling.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 353 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetPTEN
Antigen Species
Human
Gene ID5728
Gene Symbol
PTEN
Gene Name
phosphatase and tensin homolog
Uniprot IDP60484
Protein Name
Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
Alternate Names
10q23del • BZS • CWS1 • DEC • GLM2 • MHAM • mitochondrial phosphatase and tensin protein alpha • mitochondrial PTENalpha • MMAC1 • MMAC1 phosphatase and tensin homolog deleted on chromosome 10 • mutated in multiple advanced cancers 1 • Phosphatase and tensin homolog • phosphatase and tensin-like protein • phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN • phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN • protein tyrosine phosphatase • PTEN1 • PTENbeta • TEP1
Product Information
Applications
IHC, IHC-IF
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to PTEN immobilized on solid support.
The epitope recognized by IHC-00358 maps to a region between residue 350 and the C-terminus (residue 403) of human phosphatase and tensin homolog using the numbering given in entry NP_000305.2 (GeneID 5728).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Immunohistochemistry-Immunofluoresence (IHC-IF)1:50 - 1:500
Documents
Safety Data SheetPDF · IHC-00358Open
Product DatasheetPDF · IHC-00358 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00358 · Lot 1 (current lot)Open
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