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Rabbit anti-PUF60 Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
PUF60
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
between 509 and 559
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (200 µg/ml)

100 µl (10 blots)

20 µl (2 blots)

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Product Details

Specifications
Verified Reactivity
Mouse, Human
Presumed Reactivity
Bovine, Orangutan, Rat
Antigen Species
Human
Stock Concentration
40 µg/ml
200 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to PUF60 immobilized on solid support.

The epitope recognized by A302-819A-T maps to a region between residue 509 and 559 of human Poly-U Binding Splicing Factor 60KDa using the numbering given in entry NP_510965.1 (GeneID 22827).

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Gene & Protein Information

Poly-U binding splicing factor 60KDa (PUF60) is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. PUF60 is implicated in the xeroderma pigmentosum disorder [taken from NCBI Entrez Gene (Gene ID: 22827)].

22827

PUF60

poly(U) binding splicing factor 60

Q9UHX1

Poly(U)-binding-splicing factor PUF60

Alternate Names

60 kDa poly(U)-binding-splicing factor, FBP interacting repressor, FBP-interacting repressor, FIR, FUSE-binding protein-interacting repressor, poly(U) binding splicing factor 60KDa, poly(U)-binding-splicing factor PUF60, pyrimidine tract binding splicing factor, Ro ribonucleoprotein-binding protein 1, Ro-binding protein 1, RoBP1, RoBPI, Siah binding protein 1, Siah-binding protein 1, SIAHBP1, Siah-BP1, VRJS

Applications

Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.