Bethyl Laboratories
Rabbit anti-Pygo1 Antibody Affinity Purified
Primary
Catalog #A302-648A
TargetPygo1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP
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Product Description
Pygo1 (pygopus homolog 1) is the mammalian homolog of the drosophila pygopus protein. Pygo1 is involved in signal transduction through the Wnt pathway.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 300 and 350
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetPygo1
Antigen Species
Human
Gene ID26108
Gene Symbol
PYGO1
Gene Name
pygopus family PHD finger 1
Uniprot IDQ9Y3Y4
Protein Name
Pygopus homolog 1
Alternate Names
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Pygo1 immobilized on solid support.
The epitope recognized by A302-648A maps to a region between residue 300 and 350 of human pygopus homolog 1 using the numbering given in entry NP_056432.1 (GeneID 26108 ).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-648A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-648A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-648AOpen
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