Bethyl Laboratories
Rabbit anti-RABGEF1 Antibody Affinity Purified
Primary
Catalog #A302-922A
TargetRABGEF1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP
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Product Description
RabGEF1 (Rab guanine nucleotide exchange factor 1) is a rab effector protein acting as linker between gamma-adaptin, Rab4A or Rab5A. RabGEF1 is involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. It stimulates nucleotide exchange on Rab5A. RabGEF1 can act as a ubiquitin ligase [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q9UJ41].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 275 and 325
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetRABGEF1
Antigen Species
Human
Gene ID27342
Gene Symbol
RABGEF1
Gene Name
RAB guanine nucleotide exchange factor (GEF) 1
Uniprot IDQ9UJ41
Protein Name
Rab5 GDP/GTP exchange factor
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to RABGEF1 immobilized on solid support.
The epitope recognized by A302-922A maps to a region between residue 475 and 525 of human RAB Guanine Nucleotide Exchange Factor (GEF) 1 using the numbering given in entry BAC87138.1 (GeneID 27342).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:1000 - 1:5000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-922A · Lot 2 (current lot)Open
Product DatasheetPDF · A302-922A · Lot 2 (current lot)Open
Safety Data SheetPDF · A302-922AOpen
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