/ Antibodies & Antisera / Primary Antibodies / Rabbit anti-Rad18 Antibody
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Primary Antibodies  

Rabbit anti-Rad18 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
Rad18
Reactivity:
Human
Applications:
IP
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 200 and 250
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$121.00 $433.00
Qty:
Bulk Request

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Request Formulation Change
Country of Origin
USA

Additional Product Information

Rad18 is an E3 ubiquitin-protein ligase that associates with the E2 ubiquitin conjugating enzyme UBE2B (Rad6b) and monoubiquitinates PCNA on stalled replication forks. This activity promotes the recruitment of DNA repair polymerases such as DNA polymerase eta (Poleta) and translesion synthesis at DNA lesions.

56852
RAD18
RAD18, E3 ubiquitin protein ligase
Q9NS91
E3 ubiquitin-protein ligase RAD18

Alternate Names

E3 ubiquitin-protein ligase RAD18; hHR18; hRAD18; postreplication repair protein hRAD18p; postreplication repair protein RAD18; RAD18 homolog; RAD18, S. cerevisiae, homolog; RING finger protein 73; RING-type E3 ubiquitin transferase RAD18; RNF73

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.