Bethyl Laboratories
Rabbit anti-Rad18 Antibody Affinity Purified
Primary
Catalog #A301-339A
TargetRad18
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
Rad18 is an E3 ubiquitin-protein ligase that associates with the E2 ubiquitin conjugating enzyme UBE2B (Rad6b) and monoubiquitinates PCNA on stalled replication forks. This activity promotes the recruitment of DNA repair polymerases such as DNA polymerase eta (Poleta) and translesion synthesis at DNA lesions.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 200 and 250
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetRad18
Antigen Species
Human
Gene ID56852
Gene Symbol
RAD18
Gene Name
RAD18, E3 ubiquitin protein ligase
Uniprot IDQ9NS91
Protein Name
E3 ubiquitin-protein ligase RAD18
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Rad18 immobilized on solid support.
The epitope recognized by A301-339A maps to a region between residue 175 and 225 of human E3 ubiquitin-protein ligase RAD18 using the numbering given in entry NP_064550.2 (GeneID 56852).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-339A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-339A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-339AOpen
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