Bethyl Laboratories
Rabbit anti-RAD51C Antibody Affinity Purified
Primary
Catalog #A302-645A
TargetRAD51C
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationWB
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Product Description
RAD51C (RAD51 homolog C) is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. The RAD51B-RAD51C dimer exhibits single-stranded DNA-dependent ATPase activity. RAD51C is part of the BCDX2 complex. The BCDX2 complex binds single-stranded DNA, single-stranded gaps in duplex DNA, and nicks in duplex DNA [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O43502].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 326 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetRAD51C
Antigen Species
Human
Gene ID5889
Gene Symbol
RAD51C
Gene Name
RAD51 paralog C
Uniprot IDO43502
Protein Name
DNA repair protein RAD51 homolog 3
Alternate Names
Product Information
Applications
WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to RAD51C immobilized on solid support.
The epitope recognized by A302-645A maps to a region between residue 326 and 376 of human RAD51 homolog C using the numbering given in entry NP_478123.1 (GeneID 5889).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-645A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-645A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-645AOpen
Citations / Publications
Services
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