Bethyl Laboratories
Rabbit anti-Rad9 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00376
TargetRad9
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IHC-IF
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Product Description
Rad9 is a cell-cycle checkpoint protein with 3’-5’ exonuclease activity and is part of the 9-1-1 complex. The 9-1-1 complex, also called the checkpoint clamp, is a trimeric complex that includes Rad9, Hus1, and Rad1. This complex is recruited by the RAD17/RFC clamp loader to DNA lesions where it aids in the activation of Chk2 by ATM and ATRIP and the subsequent triggering of the checkpoint signaling cascade. In addition to its role in sensing DNA damage, Rad9 also functions in the base excision repair process of DNA repair.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 341 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetRad9
Antigen Species
Human
Gene ID5883
Gene Symbol
RAD9A
Gene Name
RAD9 checkpoint clamp component A
Uniprot IDQ99638
Protein Name
Cell cycle checkpoint control protein RAD9A
Alternate Names
Product Information
Applications
IHC, IHC-IF
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Rad9 immobilized on solid support.
The epitope recognized by IHC-00376 maps to a region between residue 350 and the C-terminus (residue 391) of human Rad9 using the numbering given in entry NP_004575.1 (GeneID 5883).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Immunohistochemistry-Immunofluoresence (IHC-IF)1:50 - 1:500
Documents
Safety Data SheetPDF · IHC-00376Open
Product DatasheetPDF · IHC-00376 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00376 · Lot 1 (current lot)Open
Citations / Publications
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