/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-RAF1/c-Raf Recombinant Monoclonal Antibody [BLR289L]
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Primary Antibodies  

Rabbit anti-RAF1/c-Raf Recombinant Monoclonal Antibody [BLR289L]

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

RAF1/c-Raf

Reactivity:

Human

,

Mouse

Applications:

Flow Cyt

,

IP

,

WB

Host:

Rabbit

Clonality:

Recombinant Monoclonal

Clone:

BLR289L

Format:

Whole IgG

Immunogen:

between 300 and 350

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Purified

100 µl (50+ tests)

10 µl (5+ tests)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free


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Additional Product Information

RAF1 is a mitogen activated kinase kinase kinase (MAPKKK). In response to extracellular signals, RAF1 phosphorylates the MEK1 and MEK2 MAP kinase kinases. MEK1 and MEK2 subsequently phosphorylate and activate the ERK1 and ERK2 MAP kinases to exert pleiotropic effects on cellular functions such as growth, division, differentiation, motility, and apoptosis. RAF1 associates with the Ras family of membrane GTPases and functions downstream of Ras activity.

5894

RAF1

Raf-1 proto-oncogene, serine/threonine kinase

P04049

RAF proto-oncogene serine/threonine-protein kinase

Alternate Names

CMD1NN;CRAF;c-Raf;C-Raf proto-oncogene, serine/threonine kinase;NS5;Oncogene RAF1;proto-oncogene c-RAF;raf proto-oncogene serine/threonine protein kinase;RAF proto-oncogene serine/threonine-protein kinase;Raf-1;v-raf-1 murine leukemia viral oncogene homolog 1;v-raf-1 murine leukemia viral oncogene-like protein 1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.