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Rabbit anti-RanBP1 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
RanBP1
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 151 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to RanBP1 immobilized on solid support.

The epitope recognized by A300-512A maps to a region between residues 150 and the C-terminus (residue 201) of human RAN Binding Protein 1 using the numbering given in entry NP_002873.1 (GeneID 5902).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to RanBP1 immobilized on solid support.

The epitope recognized by A300-512A-T maps to a region between residues 150 and the C-terminus (residue 201) of human RAN Binding Protein 1 using the numbering given in entry NP_002873.1 (GeneID 5902).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

Ran-binding protein 1 (RanBP1) binds to the GTP-binding protein, Ran, when it is specifically complexed with GTP. RanBP1 binding to GTP-charged RAN increases the catalytic efficiency of GTP hydrolysis activated by RanGAP, the Ran specific GTPase-activating protein. RanBP1 is also a negative regulator of RCC1 which functions in the nuclear pore transport process and cell cycle progression.

5902
RANBP1
RAN binding protein 1
P43487
Ran-specific GTPase-activating protein

Alternate Names

HpaII tiny fragments locus 9A; HTF9A; Ran-binding protein 1; RanBP1; ran-specific GTPase-activating protein; testis secretory sperm-binding protein Li 221n

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.