Bethyl Laboratories
Rabbit anti-RanBP2 Antibody Affinity Purified
Primary
Catalog #A301-796A
TargetRanBP2
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
Ran binding protein 2 (RanBP2) is a large multi-modular nucleoporin protein (also called NUP358) that bears several structural domains including a cyclophilin-type rotamase domain, four RanBD1 domains, eight RanBP2-type zinc fingers, and one TPR repeat. This variety of structural domains imparts pleiotropic qualities to RanBP2. RanBP2 has been shown to function as an E3 SUMO-protein ligase and is also part of the nuclear export pathway.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1050 and 1100
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetRanBP2
Antigen Species
Human
Gene ID5903
Gene Symbol
RANBP2
Gene Name
RAN binding protein 2
Uniprot IDP49792
Protein Name
E3 SUMO-protein ligase RanBP2
Alternate Names
358 kDa nucleoporin • acute necrotizing encephalopathy 1 (autosomal dominant) • ADANE • ANE1 • E3 SUMO-protein ligase RanBP2 • E3 SUMO-protein transferase RanBP2 • IIAE3 • nuclear pore complex protein Nup358 • nucleoporin 358 • nucleoporin Nup358 • NUP358 • P270 • ran-binding protein 2 • RanBP2 • transformation-related protein 2 • TRP1 • TRP2
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to RanBP2 immobilized on solid support.
The epitope recognized by A301-796A maps to a region between residue 1050 and 1100 of human RAN binding protein 2 using the numbering given in entry NP_006258.3 (GeneID 5903).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000 (3-8% Tris-acetate gel recommended)
Documents
Product DatasheetPDF · A301-796A · Lot 3 (current lot)Open
Product DatasheetPDF · A301-796A · Lot 3 (current lot)Open
Safety Data SheetPDF · A301-796AOpen
Citations / Publications
Services
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