/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-RanBP3 Antibody
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Primary Antibodies  

Rabbit anti-RanBP3 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

RanBP3

Reactivity:

Human

,

Mouse

Applications:

IHC-IF

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 150 and 200

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to RanBP3 immobilized on solid support.

The epitope recognized by A301-520A maps to a region between residue 150 and 200 of human RAN binding protein 3 using the numbering given in entry NP_015561.1 (GeneID 8498).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to RanBP3 immobilized on solid support.

The epitope recognized by A301-520A-T maps to a region between residue 150 and 200 of human RAN binding protein 3 using the numbering given in entry NP_015561.1 (GeneID 8498).

Additional Product Information

Ran binding protein 3 (RanBP3) is a Ran (ras-related nuclear protein) binding protein and a component of a nuclear transport complex that functions to export proteins out of the nucleus. RanBP3 binds the nucleocytoplasmic transport receptor, CRM1, and facilitates the export of CRM1 substrates. Recently it has been shown that RanBP3 function is regulated by the Ras/ERK/RSK and PI3K/Akt signaling pathway. This finding has provided a link between nuclear transport, cell signaling, and cell fate.

8498

RANBP3

RAN binding protein 3

Q9H6Z4

Ran-binding protein 3

Alternate Names

epididymis secretory sperm binding protein; ran-binding protein 3; RanBP3

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.