Bethyl Laboratories
Rabbit anti-RanGAP1 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00586
TargetRanGAP1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
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Product Description
The regulation of GTPase activity is achieved via activators called GEFs (guanine-nucleotide exchange factors) and inactivators called GAPs (GTPase-activating proteins). Ran-specific GTPase-activating protein 1 (RanGAP1) induces the GTPase activity of the RAN small GTPase. The Ran GTPase plays a role in spatial information and nucleocytoplasmic transport. RanGAP1 is the antagonist of the GEF RCC1 which keeps RAN in the active GTP-bound state.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 400 and 450
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetRanGAP1
Antigen Species
Human
Gene ID5905
Gene Symbol
RANGAP1
Gene Name
Ran GTPase activating protein 1
Uniprot IDP46060
Protein Name
Ran GTPase-activating protein 1
Alternate Names
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to RanGAP1 immobilized on solid support.
The epitope recognized by IHC-00586 maps to a region between residue 400 and 450 of human Ran GTPase activating protein 1 using the numbering given in entry NP_002874.1 (GeneID 5905).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer or tris-EDTA pH9.0 buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00586Open
Product DatasheetPDF · IHC-00586 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00586 · Lot 1 (current lot)Open
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