Bethyl Laboratories
Rabbit anti-RANK Antibody Affinity Purified
Primary
Catalog #A303-807A
TargetRANK
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
RANK (receptor activator of NF-KB) is a member of the TNF-receptor superfamily. TNF-receptor family members can interact with various TRAF family proteins, through which this receptor induces the activation of NF-kappa B and MAPK8/JNK. RANK and its ligand are important regulators of the interaction between T cells and dendritic cells. RANK is also an essential mediator for osteoclast and lymph node development. Studies of the mouse counterpart suggest that this receptor directly mediates the osteoprotegerin ligand (OPGL)-induced osteoclastogenesis in osteoclast precursor cells [taken from NCBI Entrez Gene (Gene ID: 8792)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 450 and 500
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetRANK
Antigen Species
Human
Gene ID8792
Gene Symbol
TNFRSF11A
Gene Name
TNF receptor superfamily member 11a
Uniprot IDQ9Y6Q6
Protein Name
Tumor necrosis factor receptor superfamily member 11A
Alternate Names
CD antigen CD265 • CD265 • FEO • LOH18CR1 • loss of heterozygosity, 18, chromosomal region 1 • ODFR • OFE • OPTB7 • osteoclast differentiation factor receptor • OSTS • Paget disease of bone 2 • PDB2 • RANK • receptor activator of NF-KB • receptor activator of nuclear factor-kappa B • TRANCER • tumor necrosis factor receptor superfamily member 11A • tumor necrosis factor receptor superfamily, member 11a, NFKB activator
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to RANK immobilized on solid support.
The epitope recognized by A303-807A maps to a region between residue 450 and 500 of human Receptor activator of NF-KB using the numbering given in entry NP_003830.1 (GeneID 8792).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:1000 - 1:5000
Documents
Product DatasheetPDF · A303-807A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-807A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-807AOpen
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