Bethyl Laboratories
Rabbit anti-RAP74 Antibody Affinity Purified
Primary
Catalog #A302-757A
TargetRAP74
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
The general transcription factor, TFIIF, is composed of a heterodimer of a 74 kDa alpha subunit and a 30 kDa beta subunit. The alpha subunit of TFIIF is also called RAP74. In collaboration with TFIIB, TFIIF helps recruit RNA polymerase II to the pre-initiation complex (PIC).
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 350 and 400
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetRAP74
Antigen Species
Human
Gene ID2962
Gene Symbol
GTF2F1
Gene Name
general transcription factor IIF subunit 1
Uniprot IDP35269
Protein Name
General transcription factor IIF subunit 1
Alternate Names
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to RAP74 immobilized on solid support.
The epitope recognized by A302-757A maps to a region between residue 350 and 400 of human Transcription Initiation Factor RAP74 using the numbering given in entry NP_002087.2 (GeneID 2962).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-757A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-757A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-757AOpen
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