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Primary Antibodies  

Rabbit anti-RAP80 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Citations (12)
Target:

RAP80

Reactivity:

Human

Applications:

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 650 and C-term

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

RAP80 was first identified as a nuclear protein that interacts with the retinoid-related testis-associated receptor (RTR) and is able to inhibit the interaction of RTR with the N-CoR co-repressor. RAP80 has also been shown to interact with estrogen receptor alpha (ER-alpha) and modulate its activity. The modulation of ER-alpha activity is dependent on the ubiquitin-interacting motif (UIM) of RAP80. The UIM of RAP80 has also been demonstrated to be important for the BRCA1-dependent DNA repair response.

51720

UIMC1

ubiquitin interaction motif containing 1

Q96RL1

BRCA1-A complex subunit RAP80

Alternate Names

BRCA1-A complex subunit RAP80; RAP80; receptor-associated protein 80; retinoid X receptor-interacting protein 110; Ubiquitin interaction motif-containing protein 1; X2HRIP110

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.