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Rabbit anti-RASAL1 Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
RASAL1
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
between 754 and 804
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (10 blots)

20 µl (2 blots)

For ordering information, see our International Distributors

Product Details

Specifications
Verified Reactivity
Human
Antigen Species
Human
Stock Concentration
40 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to RASAL1 immobilized on solid support.

The epitope recognized by A305-876A-T maps to a region between residue 754 and 804 of human RasGAP-activating-like protein 1 using the numbering given in entry O95294.3 (GeneID 8437).

Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

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Gene & Protein Information

RASAL1 is a member of the GAP1 family of GTPase-activating proteins. These proteins stimulate the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, RASAL1 enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. This particular family member contains domains which are characteristic of the GAP1 subfamily of RasGAP proteins but, in contrast to the other GAP1 family members, RASAL1 is strongly and selectively expressed in endocrine tissues. Alternatively spliced transcript variants that encode different isoforms have been described [provided by RefSeq, Jul 2010] [taken from NCBI Entrez Gene (Gene ID: 8437)].

8437

RASAL1

RAS protein activator like 1

O95294

RasGAP-activating-like protein 1

Alternate Names

GAP1 like protein, ras GTPase-activating-like protein, RAS protein activator like 1, RASAL, rasGAP-activating-like protein 1

Applications

Western blot of immunoprecipitates performed using Normal Pig Serum (Cat. No. S100-020), Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) and 4-8% SDS-PAGE. Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.