Bethyl Laboratories
Rabbit anti-RbBP7 Antibody Affinity Purified
Primary
Catalog #A300-958A
TargetRbBP7
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
Retinoblastoma-binding protein 7 (RbBP7) is a component of several complexes that regulate chromatin remodeling and assembly. RbBP7 is a subunit of the histone acetyltransferase (HAT) complex and of the core mSin3 histone deacetylase (HDAC) complex. RbBP7 is a histone binding subunit that functions to recruit chromatin assembly and remodeling factors to histones. RbBP7 also interacts with pRb and BRCA1 to modulate their activity.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetRbBP7
Antigen Species
Human
Gene ID5931
Gene Symbol
RBBP7
Gene Name
RB binding protein 7, chromatin remodeling factor
Uniprot IDQ16576
Protein Name
Histone-binding protein RBBP7
Alternate Names
G1/S transition control protein-binding protein RbAp46 • histone acetyltransferase type B subunit 2 • histone-binding protein RBBP7 • nucleosome-remodeling factor subunit RBAP46 • RbAp46 • RBBP-7 • retinoblastoma-binding protein 7 • retinoblastoma-binding protein p46 • retinoblastoma-binding protein RbAp46
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to RbBP7 immobilized on solid support.
The epitope recognized by A300-958A maps to a region between residue 1 and 50 of human Retinoblastoma Binding Protein 7 using the numbering given in entry AAC50231.1 (GeneID 5931).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:200 - 1:1000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A300-958A · Lot 1 (current lot)Open
Product DatasheetPDF · A300-958A · Lot 1 (current lot)Open
Safety Data SheetPDF · A300-958AOpen
Citations / Publications
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