Bethyl Laboratories
Rabbit anti-RBM5 Antibody Affinity Purified
Primary
Catalog #A302-228A
TargetRBM5
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
RNA-binding motif protein 5 (RBM5) is also known as LUCA-15. The RBM5 gene is located within a region highly susceptible to deletions in lung cancer and is implicated to be a tumor suppressor. RBM5 bears two RRM domains, one RanBP2-type zinc finger, one C2H2-type zinc finger, and a G-patch domain. It has been found to be a component of the spliceosome, a complex that regulates alternative splicing and is involved in the regulation of proliferation and apoptosis.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 765 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetRBM5
Antigen Species
Human
Gene ID10181
Gene Symbol
RBM5
Gene Name
RNA binding motif protein 5
Uniprot IDP52756
Protein Name
RNA-binding protein 5
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to RBM5 immobilized on solid support.
The epitope recognized by A302-228A maps to a region between residue 765 and 815 of human RNA binding motif protein 5 using the numbering given in entry NP_005769.1 (GeneID 10181).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-228A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-228A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-228AOpen
Citations / Publications
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