Bethyl Laboratories
Rabbit anti-RBM9 Antibody Affinity Purified
Primary
Catalog #A300-864A
TargetRBM9
HostRabbit
ClonalityPolyclonal
ReactivityRat, Mouse, Human
ApplicationFlow Cyt, IHC, IP, WB
Image 1 of 5
local_shipping
Order within 14 hours and 16 minutes for shipment by today
Product Description
RBM9 (RNA binding motif protein 9) is a member of the Fox family of pre-mRNA splicing regulatory proteins. RBM9, also known as Fox-2, has been identified as an important regulator of tissue-specific alternative splicing that functions as a switch to activate intron inclusion during cell and tissue development. RBM9 has also been identified as a negative coregulator of the estrogen receptor (ER).
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Rat, Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetRBM9
Antigen Species
Human
Gene ID23543
Gene Symbol
RBFOX2
Gene Name
RNA binding protein, fox-1 homolog 2
Uniprot IDO43251
Protein Name
RNA binding protein fox-1 homolog 2
Alternate Names
dJ106I20.3 • fox-1 homolog B • fox-1 homologue • FOX2 • Fox-2 • fxh • hexaribonucleotide-binding protein 2 • HNRBP2 • HRNBP2 • RBM9 • repressor of tamoxifen transcriptional activity • RNA binding protein fox-1 homolog 2 • RNA binding protein, fox-1 homolog 2 • RNA-binding motif protein 9 • RNA-binding protein 9 • RTA
Product Information
Applications
Flow Cyt, IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to FOX2/RBM9 immobilized on solid support.
The epitope recognized by A300-864A maps to a region between residue 1 and 50 of human RNA Binding Motif Protein 9 using the numbering given in entry NP_055124.1 (GeneID 23543).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Flow Cytometry (Flow Cyt)Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.25 µl per 1 x 10^6 cells.
Immunohistochemistry (IHC)1:2000 to 1:10,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A300-864A · Lot 4 (current lot)Open
Product DatasheetPDF · A300-864A · Lot 4 (current lot)Open
Safety Data SheetPDF · A300-864AOpen
Citations / Publications
Related Products
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request Customization
Custom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal Development
VHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH Discovery
Resources
Standard Western Blot Protocol
Read the Protocol
Immunoprecipitation Protocol
Read the Protocol
Simple Western™ Resources
Explore Simple Western™
1