/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-RCAS1 Antibody
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Primary Antibodies  

Rabbit anti-RCAS1 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

RCAS1

Reactivity:

Human

,

Mouse

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 163 and 213

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Additional Product Information

The gene for RCAS1 (receptor-binding cancer antigen expressed on SiSo cells) was identified as an estrogen-responsive gene. Regulation by estrogen of transcription of RCAS1 is mediated by the estrogen receptor which binds to the estrogen-responsive element (ERE) found in the 5'-flanking region of the RCAS1 gene, EBAG9 (estrogen receptor binding site associated antigen 9). RCAS1 is a tumor-associated antigen that is expressed at high frequency in a variety of cancers [taken from NCBI Entrez Gene (GeneID: 9166)].

9166

EBAG9

estrogen receptor binding site associated, antigen, 9

O00559

Receptor-binding cancer antigen expressed on SiSo cells

Alternate Names

cancer-associated surface antigen RCAS1; EB9; estrogen receptor-binding fragment-associated gene 9 protein; PDAF; receptor-binding cancer antigen expressed on SiSo cells

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.