Bethyl Laboratories
Rabbit anti-RCH1/KPNA2 Antibody Affinity Purified
Primary
Catalog #A300-484A
TargetRCH1/KPNA2
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
The import of proteins from the cytoplasm to the nucleus involves docking the protein to receptors associated with the nuclear pore complex followed by translocation through the nuclear pore. RCH1 is an adaptor protein that recognizes the nuclear localization signal (NLS) on cargo and binds the karyopherin importin-beta receptor for nuclear import which is regulated by RanGTP.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 479 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetRCH1/KPNA2
Antigen Species
Human
Gene ID3838
Gene Symbol
KPNA2
Gene Name
karyopherin subunit alpha 2
Uniprot IDP52292
Protein Name
Importin subunit alpha-1
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to RCH1/KPNA2 immobilized on solid support.
The epitope recognized by A300-484A maps to a region between residue 475 and the C-terminus (residue 529) of human RAG cohort 1 (Karyopherin Alpha 2) using the numbering given in entry NP_002257.1 (GeneID 3838).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:1000 to 1:5000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:10,000 - 1:25,000
Documents
Product DatasheetPDF · A300-484A · Lot 3 (current lot)Open
Product DatasheetPDF · A300-484A · Lot 3 (current lot)Open
Safety Data SheetPDF · A300-484AOpen
Citations / Publications
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