Bethyl Laboratories
Rabbit anti-REEP3 Antibody Affinity Purified
Primary
Catalog #A305-662A
TargetREEP3
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
Receptor expression-enhancing protein 3 (REEP3) is a microtubule-binding protein required to ensure proper cell division and nuclear envelope reassembly by sequestering the endoplasmic reticulum away from chromosomes during mitosis. REEP3 probably acts by clearing the endoplasmic reticulum membrane from metaphase chromosomes [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q6NUK4].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 205 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetREEP3
Antigen Species
Human
Gene ID221035
Gene Symbol
REEP3
Gene Name
receptor accessory protein 3
Uniprot IDQ6NUK4
Protein Name
Receptor expression-enhancing protein 3
Alternate Names
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to REEP3 immobilized on solid support.
The epitope recognized by A305-662A maps to a region between residue 205 to 255 of human Receptor expression-enhancing protein 3 using the numbering given in entry Q6NUK4.1 (GeneID 221035).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A305-662A · Lot 1 (current lot)Open
Product DatasheetPDF · A305-662A · Lot 1 (current lot)Open
Safety Data SheetPDF · A305-662AOpen
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