Rabbit anti-RENT2 Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
Regulator of nonsense transcripts 2 (RENT2), also known as UPF2, is involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC). RENT2 is recruited by UPF3B associated with the EJC core at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. In cooperation with UPF3B, RENT2 stimulates both ATPase and RNA helicase activities of UPF1. RENT2 binds spliced mRNA [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9HAU5].
Alternate Names
FRS2/UPF2/LEMD3 fusion; HUPF2; LEMD3/UPF2 fusion; nonsense mRNA reducing factor 2; regulator of nonsense transcripts 2; RENT2; smg-3; smg-3 homolog, nonsense mediated mRNA decay factor; UPF2 regulator of nonsense transcripts homolog; up-frameshift suppressor 2 homolog; yeast Upf2p homolog
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.