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Rabbit anti-REST Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
REST
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
between 800 and 850
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

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Product Details

Specifications
Verified Reactivity
Human
Antigen Species
Human
Stock Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to REST immobilized on solid support.

The epitope recognized by A300-539A-T maps to a region between residues 800 and 850 of human RE1-silencing transcription factor using the numbering given in entry NP_005603.2 (GeneID 5978).

Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

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Gene & Protein Information

RE1-Silencing Transcription Factor (REST) is a transcriptional repressor that silences neuronal gene expression in non-neuronal cells by binding to the neuron-restrictive silencer element (NRSE). REST is a Kruppel-type zinc finger transcription factor that mediates repression through its interaction with the mSin3 and CoREST corepressors and the HDAC1 and HDAC2 histone deacetylases.

5978

REST

RE1 silencing transcription factor

Q13127

RE1-silencing transcription factor

Alternate Names

DFNA27, GINGF5, HGF5, neural-restrictive silencer factor, neuron restrictive silencer factor, NRSF, RE1-silencing transcription factor, repressor binding to the X2 box, WT6, X2 box repressor, XBR

Applications

Western blot of immunoprecipitates performed using Normal Pig Serum (Cat. No. S100-020), Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) and 4-8% SDS-PAGE.