Bethyl Laboratories
Rabbit anti-REST Antibody Affinity Purified
Primary
Catalog #A300-539A
TargetREST
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP
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Product Description
RE1-Silencing Transcription Factor (REST) is a transcriptional repressor that silences neuronal gene expression in non-neuronal cells by binding to the neuron-restrictive silencer element (NRSE). REST is a Kruppel-type zinc finger transcription factor that mediates repression through its interaction with the mSin3 and CoREST corepressors and the HDAC1 and HDAC2 histone deacetylases.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 800 and 850
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetREST
Antigen Species
Human
Gene ID5978
Gene Symbol
REST
Gene Name
RE1 silencing transcription factor
Uniprot IDQ13127
Protein Name
RE1-silencing transcription factor
Alternate Names
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to REST immobilized on solid support.
The epitope recognized by A300-539A maps to a region between residues 800 and 850 of human RE1-silencing transcription factor using the numbering given in entry NP_005603.2 (GeneID 5978).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A300-539A · Lot 2 (current lot)Open
Product DatasheetPDF · A300-539A · Lot 2 (current lot)Open
Safety Data SheetPDF · A300-539AOpen
Citations / Publications
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