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Rabbit anti-RFC1 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

RFC1

Reactivity:

Human

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 1098 and C-terminus

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$121.00 $433.00
Qty:

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to RFC1 immobilized on solid support.

The epitope recognized by A300-320A maps to a region between residue 1100 and the C-terminus (residue 1148) of human Replication Factor C subunit 1 using the numbering given in entry NP_002904.3 (GeneID 5981).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to RFC1 immobilized on solid support.

The epitope recognized by A300-320A-T maps to a region between residue 1100 and the C-terminus (residue 1148) of human Replication Factor C subunit 1 using the numbering given in entry NP_002904.3 (GeneID 5981).

Country of Origin

USA

Additional Product Information

The Replication factor C (RFC) heteropentamer includes RFC1, RFC2, RFC3, RFC4, and RFC5. The RFC heteropentamer is part of the multiprotein DNA replicase that functions to replicate DNA prior to cell division and repair DNA damage. RFC is the clamp loader ATPase associated with the DNA replicase. As the clamp loader, RFC uses ATP to recruit, open, and close the PCNA ring to link it to DNA for replication and repair. RFC1 is the 140 kDa subunit of RFC that has a DNA-binding domain with a BRCT motif and is suggested to play a more critical role than the other subunits.

5981

RFC1

replication factor C subunit 1

P35251

Replication factor C subunit 1

Alternate Names

A1; A1 140 kDa subunit; activator 1 140 kDa subunit; activator 1 large subunit; activator 1 subunit 1; DNA-binding protein PO-GA; MHC binding factor, beta; MHCBFB; PO-GA; RECC1; replication factor C (activator 1) 1, 145kDa; replication factor C 140 kDa subunit; replication factor C large subunit; replication factor C subunit 1; replication factor C1; RFC; RF-C 140 kDa subunit; RFC140

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).