Bethyl Laboratories
Rabbit anti-RFX1 Antibody Affinity Purified
Primary
Catalog #A303-042A
TargetRFX1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
Regulatory factor X, 1 (RFX1) is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X2, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with RFX family members X2, X3, and X5, but not with X4. This protein binds to the X-boxes of MHC class II genes and is essential for their expression [taken from NCBI Entrez Gene (Gene ID: 5989)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 200 and 250
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetRFX1
Antigen Species
Human
Gene ID5989
Gene Symbol
RFX1
Gene Name
regulatory factor X1
Uniprot IDP22670
Protein Name
MHC class II regulatory factor RFX1
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to RFX1 immobilized on solid support.
The epitope recognized by A303-042A maps to a region between residue 200 and 250 of human Regulatory Factor X, 1 using the numbering given in entry NP_002909.4 (GeneID 5989).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A303-042A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-042A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-042AOpen
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