Bethyl Laboratories
Rabbit anti-RFX5 Antibody Affinity Purified
Primary
Catalog #A300-030A
TargetRFX5
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
Regulatory factor X-5 (RFX5) is a 75kDa subunit of the regulatory factor X (RFX) heteromeric complex that activates the transcription of major histocompatibility class II (MHC-II) genes by binding MHC-II promoter elements. The RFX complex consists of RFX5, RFXANK, and RFXAP. Mutations in these genes result in a lack of MHCII transcription and lead to the severe combined immunodeficiency disease, Bare Lymphocyte Syndrome (BLS).
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 566 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetRFX5
Antigen Species
Human
Gene ID5993
Gene Symbol
RFX5
Gene Name
regulatory factor X5
Uniprot IDP48382
Protein Name
DNA-binding protein RFX5
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CSN3 immobilized on solid support.
The epitope recognized by A300-030A maps to a region between residue 575 and the C-terminus (residue 616) of human Regulatory Factor X, 5 using the numbering given in entry NP_000440.1 (GeneID 5993).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:500 - 1:5000
Documents
Product DatasheetPDF · A300-030A · Lot 1 (current lot)Open
Product DatasheetPDF · A300-030A · Lot 1 (current lot)Open
Safety Data SheetPDF · A300-030AOpen
Citations / Publications
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