Bethyl Laboratories
Rabbit anti-Ric-8A Antibody Affinity Purified
Primary
Catalog #A304-571A
TargetRic-8A
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
RIC8 guanine nucleotide exchange factor A (Ric-8A), also known as Synmebryn-A, is a guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. It is able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Ric-8A is involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex (By similarity). It also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9NPQ8].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetRic-8A
Antigen Species
Human
Gene ID60626
Gene Symbol
RIC8A
Gene Name
RIC8 guanine nucleotide exchange factor A
Uniprot IDQ9NPQ8
Protein Name
Synembryn-A
Alternate Names
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Ric-8A immobilized on solid support.
The epitope recognized by A304-571A maps to a region between residue 1 to 50 of human Resistance to Inhibitors of Cholinesterase 8 Homolog A using the numbering given in entry EAW61245.1 (GeneID 60626).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A304-571A · Lot 1 (current lot)Open
Product DatasheetPDF · A304-571A · Lot 1 (current lot)Open
Safety Data SheetPDF · A304-571AOpen
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