Bethyl Laboratories
Rabbit anti-RIOK2 Antibody Affinity Purified
Primary
Catalog #A302-450A
TargetRIOK2
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
RIO kinase 2 (RIOK2) is a member of the RIO family of atypical serine protein kinases first characterized in yeast. There are three RIO kinase subfamilies: RIO1, RIO2, and RIO3. RIOK1 and RIOK2 proteins are present in organisms from Archaea to humans. Studies of RIO kinases in yeast indicate that they play a role in ribosome biogenesis. Human RIOK2 has been shown to be a part of a late 40S pre-ribosomal particle and have influence on 40S maturation.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 502 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetRIOK2
Antigen Species
Human
Gene ID55781
Gene Symbol
RIOK2
Gene Name
RIO kinase 2
Uniprot IDQ9BVS4
Protein Name
Serine/threonine-protein kinase RIO2
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to RIOK2 immobilized on solid support.
The epitope recognized by A302-450A maps to a region between residue 502 and 552 of human RIO kinase 2 using the numbering given in entry NP_060813.1 (GeneID 55781).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:1000 - 1:5000
Documents
Product DatasheetPDF · A302-450A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-450A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-450AOpen
Citations / Publications
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