/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-RNF138 Antibody
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Primary Antibodies  

Rabbit anti-RNF138 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

RNF138

Reactivity:

Human

Applications:

IP

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 195 and 245

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Bovine

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

RING-finger protein 138 (RNF138) is an E3 ubiquitin ligase. Together with NLK, RNF138 is involved in the ubiquitination and degradation of TCF/LEF. RNF138 also exhibits auto-ubiquitination activity in combination with UBE2K. RNF138 may act as a negative regulator in the Wnt/beta-catenin-mediated signaling pathway [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q8WVD3].

51444

RNF138

ring finger protein 138

Q8WVD3

E3 ubiquitin-protein ligase RNF138

Alternate Names

E3 ubiquitin-protein ligase RNF138; hNARF; HSD-4; NARF; nemo-like kinase associated ring finger protein; Nemo-like kinase-associated RING finger protein; NLK-associated RING finger protein; RING finger protein 138; ring finger protein 138, E3 ubiquitin protein ligase; RING-type E3 ubiquitin transferase RNF138; STRIN; testicular tissue protein Li 165

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.