Bethyl Laboratories
Rabbit anti-RNF138 Antibody Affinity Purified
Primary
Catalog #A302-994A
TargetRNF138
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
Image 1 of 1
local_shipping
Order within 18 hours and 50 minutes for shipment by Tuesday, November 17
Product Description
RING-finger protein 138 (RNF138) is an E3 ubiquitin ligase. Together with NLK, RNF138 is involved in the ubiquitination and degradation of TCF/LEF. RNF138 also exhibits auto-ubiquitination activity in combination with UBE2K. RNF138 may act as a negative regulator in the Wnt/beta-catenin-mediated signaling pathway [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q8WVD3].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 195 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetRNF138
Antigen Species
Human
Gene ID51444
Gene Symbol
RNF138
Gene Name
ring finger protein 138
Uniprot IDQ8WVD3
Protein Name
E3 ubiquitin-protein ligase RNF138
Alternate Names
E3 ubiquitin-protein ligase RNF138 • hNARF • HSD-4 • NARF • nemo-like kinase associated ring finger protein • Nemo-like kinase-associated RING finger protein • NLK-associated RING finger protein • RING finger protein 138 • ring finger protein 138, E3 ubiquitin protein ligase • RING-type E3 ubiquitin transferase RNF138 • STRIN • testicular tissue protein Li 165
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to RNF138 immobilized on solid support.
The epitope recognized by A302-994A maps to a region between residue 195 and 245 of human Ring Finger Protein 138 using the numbering given in entry NP_057355.2 (GeneID 51444).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-994A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-994A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-994AOpen
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1