Bethyl Laboratories
Rabbit anti-RNF138 Antibody Affinity Purified
Primary
Catalog #A302-994A
TargetRNF138
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
RING-finger protein 138 (RNF138) is an E3 ubiquitin ligase. Together with NLK, RNF138 is involved in the ubiquitination and degradation of TCF/LEF. RNF138 also exhibits auto-ubiquitination activity in combination with UBE2K. RNF138 may act as a negative regulator in the Wnt/beta-catenin-mediated signaling pathway [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q8WVD3].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 195 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetRNF138
Antigen Species
Human
Gene ID51444
Gene Symbol
RNF138
Gene Name
ring finger protein 138
Uniprot IDQ8WVD3
Protein Name
E3 ubiquitin-protein ligase RNF138
Alternate Names
E3 ubiquitin-protein ligase RNF138 • hNARF • HSD-4 • NARF • nemo-like kinase associated ring finger protein • Nemo-like kinase-associated RING finger protein • NLK-associated RING finger protein • RING finger protein 138 • ring finger protein 138, E3 ubiquitin protein ligase • RING-type E3 ubiquitin transferase RNF138 • STRIN • testicular tissue protein Li 165
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to RNF138 immobilized on solid support.
The epitope recognized by A302-994A maps to a region between residue 195 and 245 of human Ring Finger Protein 138 using the numbering given in entry NP_057355.2 (GeneID 51444).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-994A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-994A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-994AOpen
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