NEW!

Rabbit anti-RNF20 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
RNF20
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 1 and 50
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$121.00 $433.00
Qty:

Product Details

Specifications

Verified Reactivity
Mouse, Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Request Formulation Change
Production Procedures
Antibody was affinity purified using an epitope specific to RNF20 immobilized on solid support.

The epitope recognized by A300-714A maps to a region between residue 1 and 50 of human Ring Finger 20 using the numbering given in entry NP_062538.5 (GeneID 56254).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes. Targeting of proteins by ubiquitination occurs in three main steps that involve the activation of ubiquitin by E1 enzymes, the carrying of ubiquitin to the target protein by E2 enzymes, and the ligation of ubiquitin to the target protein by E3 enzymes. Ring finger protein 20 (RNF20) is a member of the RING type E3 ubiquitin ligases. RNF20 forms a complex with RNF40 to carry out H2B monoubiquitination in association with hPAF and the UbcH6 ubiquitin E2-conjugating enzyme. H2B monoubiquitination by RNF40/RNF20 has been shown to be associated with increased H3 methylation and gene transcription.

56254
RNF20
ring finger protein 20
Q5VTR2
E3 ubiquitin-protein ligase BRE1A

Alternate Names

BRE1; BRE1 E3 ubiquitin ligase homolog; BRE1A; BRE1-A; E3 ubiquitin-protein ligase BRE1A; hBRE1; homolog of S. cerevisiae BRE1; RING finger protein 20; RING-type E3 ubiquitin transferase BRE1A

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.