Bethyl Laboratories
Rabbit anti-RPC3 Antibody Affinity Purified
Primary
Catalog #A303-063A
TargetRPC3
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
RNA polymerase III subunit C (RPC3) encodes one of 17 subunits of the RNA polymerase III (PolIII) which synthesizes small RNAs, such as 5S rRNA and tRNA. The protein is encoded by the POLR3D gene.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 200 and 250
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetRPC3
Antigen Species
Human
Gene ID10623
Gene Symbol
POLR3C
Gene Name
RNA polymerase III subunit C
Uniprot IDQ9BUI4
Protein Name
DNA-directed RNA polymerase III subunit RPC3
Alternate Names
DNA-directed III 62 kDa polypeptide • DNA-directed RNA polymerase III subunit C • DNA-directed RNA polymerase III subunit RPC3 • polymerase (RNA) III (DNA directed) polypeptide C (62kD) • polymerase (RNA) III subunit C • RNA polymerase III 62 kDa subunit • RNA polymerase III subunit C3 • RPC3 • RPC62
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to RPC3 immobilized on solid support.
The epitope recognized by A303-063A maps to a region between residue 200 and 250 of human Polymerase (RNA) III (DNA directed) Polypeptide C (62kD) using the numbering given in entry NP_006459.3 (GeneID 10623).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A303-063A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-063A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-063AOpen
Citations / Publications
Services
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