Bethyl Laboratories

Rabbit anti-RPL18/Ribosomal Protein L18 Antibody Affinity Purified

Primary
Catalog #A305-341A
TargetRPL18/Ribosomal Protein L18
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationWB
Detection of human and mouse RPL18 by western blot.
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Product Description

60S ribosomal protein L18 (RPL18) is a member of the L18E family of ribosomal proteins and a component of the 60S subunit [taken from NCBI Entrez Gene (Gene ID: 6141)].

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 138 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
Antigen Species
Human
Gene ID6141
Gene Symbol
RPL18
Gene Name
ribosomal protein L18
Uniprot IDQ07020
Protein Name
60S ribosomal protein L18
Product Information
Applications
WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Antibody was affinity purified using an epitope specific to RPL18/Ribosomal Protein L18 immobilized on solid support.

The epitope recognized by A305-341A maps to a region between residue 138 to 188 of human 60S ribosomal protein L18 using the numbering given in entry Q07020.2 (GeneID 6141).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).

Western Blot (WB)1:2000 - 1:10,000

Documents

Product DatasheetPDF · A305-341A · Lot 1 (current lot)Open
Product DatasheetPDF · A305-341A · Lot 1 (current lot)Open
Safety Data SheetPDF · A305-341AOpen
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