Bethyl Laboratories
Rabbit anti-RPL7 Antibody Affinity Purified
Primary
Catalog #A300-740A
TargetRPL7
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationWB
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Product Description
RPL7 is a component of the 60S ribosomal subunit. Ribosomes are composed of a small 40S subunit and a large 60S subunit that function to catalyze protein synthesis. RPL7 is a member of the L30P family of ribosomal proteins and can bind both DNA and RNA in vitro. RPL7 is a cytoplasmic protein implicated to play a regulatory role in translation. It has also been identified as an auto-antigen in patients with autoimmune diseases such as lupus erythematosus.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 75 and 125
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetRPL7
Antigen Species
Human
Gene ID6129
Gene Symbol
RPL7
Gene Name
ribosomal protein L7
Uniprot IDP18124
Protein Name
60S ribosomal protein L7
Alternate Names
Product Information
Applications
WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to RPL7 immobilized on solid support.
The epitope recognized by A300-740A maps to a region between residue 75 and 125 of human Ribosomal Protein 7 using the numbering given in entry NP_000962.2 (GeneID 6129).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A300-740A · Lot 1 (current lot)Open
Product DatasheetPDF · A300-740A · Lot 1 (current lot)Open
Safety Data SheetPDF · A300-740AOpen
Citations / Publications
Services
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