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Rabbit anti-Rtf1 IHC Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
Rtf1
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 660 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (50+ slides)

10 µl (5+ slides)

$121.00 $459.00
Qty:

Product Details

Specifications

Verified Reactivity
Mouse, Human
Presumed Reactivity
Guinea Pig, Horse, Zebra Finch, Turkey, Dog, Chicken, Pig, Rabbit, X. tropicalis, Zebrafish, Bovine, Monkey, Rat
Antigen Species
Human
Concentration
250 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to Rtf1 immobilized on solid support.

The epitope recognized by IHC-00272 maps to the C-terminus of human Restores TBP (TATA box-binding protein) Function-1 using the numbering given in entry EAW92492.1 (GeneID 23168).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to Rtf1 immobilized on solid support.

The epitope recognized by IHC-00272-T maps to the C-terminus of human Restores TBP (TATA box-binding protein) Function-1 using the numbering given in entry EAW92492.1 (GeneID 23168).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

In yeast, Rtf1 is a component of the Paf1/RNA polymerase II complex. The Paf1 complex interacts with RNA polymerase II and functions to modify chromatin during transcription. Rtf1 appears to be important for histone H2B ubiquitination by Rad6 and for methylation of histone H3 catalyzed by the Set1/COMPASS complex that functions during transcription elongation.

23168
RTF1
RTF1 homolog, Paf1/RNA polymerase II complex component
Q92541
RNA polymerase-associated protein RTF1 homolog

Alternate Names

GTL7; KIAA0252; ortholog of mouse gene trap locus 7; RNA polymerase-associated protein RTF1 homolog; Rtf1, Paf1/RNA polymerase II complex component, homolog

Applications

Epitope exposure is recommended.

Epitope exposure with citrate buffer will enhance staining.

Likely to work with frozen sections.

In some cases, the antibody may be diluted further than indicated.