Bethyl Laboratories
Rabbit anti-SA1 Antibody Affinity Purified
Primary
Catalog #A302-578A
TargetSA1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP
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Product Description
SA1 is a component of the multisubunit cohesin complex. Cohesin functions to hold sister chromatids together during meiosis and mitosis and ensure proper segregation of chromosomes. SA1 has also been identified as a mediator of p53 induced apoptosis.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 25 and 75
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetSA1
Antigen Species
Human
Gene ID10274
Gene Symbol
STAG1
Gene Name
stromal antigen 1
Uniprot IDQ8WVM7
Protein Name
Cohesin subunit SA-1
Alternate Names
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to SA1 immobilized on solid support.
The epitope recognized by A302-578A maps to a region between residue 25 and 75 of human stromal antigen 1 using the numbering given in entry NP_005853.2 (GeneID 10274).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:2000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-578A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-578A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-578AOpen
Citations / Publications
Services
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