Bethyl Laboratories
Rabbit anti-SAE1 Antibody Affinity Purified
Primary
Catalog #A302-924A
TargetSAE1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP
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Product Description
Heterodimers of SUMO-activating enzyme subunit 1 (SAE1) and SAE2 act as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. Heterodimers mediate ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q9UBE0].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 296 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetSAE1
Antigen Species
Human
Gene ID10055
Gene Symbol
SAE1
Gene Name
SUMO1 activating enzyme subunit 1
Uniprot IDQ9UBE0
Protein Name
SUMO-activating enzyme subunit 1
Alternate Names
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to SAE1 immobilized on solid support.
The epitope recognized by A302-924A maps to a region between residue 296 and 346 of human SUMO1 Activating Enzyme Subunit 1 using the numbering given in entry NP_005491.1 (GeneID 10055).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:1000 - 1:5000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-924A · Lot 2 (current lot)Open
Product DatasheetPDF · A302-924A · Lot 2 (current lot)Open
Safety Data SheetPDF · A302-924AOpen
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