Bethyl Laboratories
Rabbit anti-SAE2 Antibody Affinity Purified
Primary
Catalog #A302-925A
TargetSAE2
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
SAE2 (SUMO-activating enzyme subunit 2) is also known as UBA2 (ubiquitin-like modifier activating enzyme 2). Heterodimers of SAE1 and SAE2 act as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. Heterodimers mediate ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q9UBT2].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 525 and 575
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetSAE2
Antigen Species
Human
Gene ID10054
Gene Symbol
UBA2
Gene Name
ubiquitin like modifier activating enzyme 2
Uniprot IDQ9UBT2
Protein Name
SUMO-activating enzyme subunit 2
Alternate Names
anthracycline-associated resistance ARX • ARX • HRIHFB2115 • SAE2 • SUMO1 activating enzyme subunit 2 • SUMO-1 activating enzyme subunit 2 • SUMO-activating enzyme subunit 2 • UBA2, ubiquitin-activating enzyme E1 homolog • ubiquitin-like 1-activating enzyme E1B • Ubiquitin-like modifier-activating enzyme 2
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to SAE2 immobilized on solid support.
The epitope recognized by A302-925A maps to a region between residue 525 and 575 of human SUMO1 Activating Enzyme Subunit 2 using the numbering given in entry NP_005490.1 (GeneID 10054).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-925A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-925A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-925AOpen
Citations / Publications
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