Bethyl Laboratories
Rabbit anti-SAH3 Antibody Affinity Purified
Primary
Catalog #A300-261A
TargetSAH3
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
SAH3 is an alkaline ceramidase isoform. Cermidases function to cleave fatty acids from ceramides to yield sphingosine. Ceramides can mediate signals that lead to differentiation, apoptosis, or senescence. SAH3 is a ceramidase isoform found in the epidermis of mammals and may play a role in keratinocyte differentiation.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetSAH3
Antigen Species
Human
Gene ID23382
Gene Symbol
AHCYL2
Gene Name
adenosylhomocysteinase like 2
Uniprot IDQ96HN2
Protein Name
Adenosylhomocysteinase 3
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to SAH3 immobilized on solid support.
The epitope recognized by A300-261A maps to a region between residues 1 and 50 of human adenosylhomocysteinase 3 (KIAA0828) using the numbering given in entry NP_056143.1 (GeneID 23382).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:1000 - 1:10,000
Documents
Product DatasheetPDF · A300-261A · Lot 1 (current lot)Open
Product DatasheetPDF · A300-261A · Lot 1 (current lot)Open
Safety Data SheetPDF · A300-261AOpen
Citations / Publications
Services
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